L-Selectin anticorps (PE)

N° du produit ABIN6657791

N° du produit (Fournisseur): 200-508-n87

Cet anticorps anti-L-Selectin est un anticorps Rat Monoclonal détectant L-Selectin dans FACS, IHC et IP. Adapté pour Souris.

Aperçu rapide pour L-Selectin anticorps (PE) (ABIN6657791)

Antigène: L-Selectin (SELL) (Selectin L (SELL))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp L-Selectin est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: MEL-14

Détail du produit anti-L-Selectin anticorps (PE)

N° du produit (Fournisseur): 200-508-n87

Fournisseur: Rockland

Fonction: CD62L Phycoerythrin Antibody

Réactivité croisée (Details): Cross reactivity with CD62L from other sources has not been tested.

Purification: Phycoerythrin conjugated CD62L Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD62L.

Stérilité: Sterile filtered

Immunogène: Anti-CD62L Antibody (Monoclonal) was produced by repeated immunizations with C3H/eb mouse B lymphoma 38C-13.

Isotype: IgG2a kappa

Labeling Ratio: 1-2

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Commentaires:

Anti-CD62L is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Date de péremption: 6 months

Détails sur L-Selectin

Antigène: L-Selectin (SELL) (Selectin L (SELL))

Autre désignation: CD62L

Sujet:

Synonyms: L-selectin CD62 antigen-like family member L Leukocyte adhesion molecule 1 LAM-1 Leukocyte-endothelial cell adhesion molecule 1 LECAM1 Lymph node homing receptor Lymphocyte antigen 22 Ly-22 Lymphocyte surface MEL-14 antigen CD62L Sell Lnhr, Ly-22, Ly22

Background: CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naive T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naive, effector, and memory T cells. CD62L has been reported to interact with CD34, glyCAM-1, and MAdCAM-1.

Gene Name: Sell

ID gène: 20343

NCBI Accession: NP_035476

UniProt: P18337